{"id":8458,"date":"2024-02-14T12:06:14","date_gmt":"2024-02-14T11:06:14","guid":{"rendered":"https:\/\/dandiag.dk\/?p=8458"},"modified":"2024-03-20T12:12:12","modified_gmt":"2024-03-20T11:12:12","slug":"vigtigt-at-vide-om-den-nye-pipette-kalibreringsstandard-iso-86552022","status":"publish","type":"post","link":"https:\/\/demo.hviiddigital.dk\/dandiag.dk\/viden\/artikler\/vigtigt-at-vide-om-den-nye-pipette-kalibreringsstandard-iso-86552022\/","title":{"rendered":"Vigtigt at vide om den nye pipette kalibreringsstandard ISO 8655:2022"},"content":{"rendered":"\n

Sartorius har samlet fem vigtige punkter omkring de seneste \u00e6ndringer i pipettekalibreringsstandarderne, som defineres i ISO 8655:2022. Disse opdateringer er fra april 2022.<\/p>\n\n\n\n

1. Hvad er ISO 8655-standarden?<\/strong><\/p>\n\n\n\n

Pipetters n\u00f8jagtighed er helt afg\u00f8rende. ISO 8655 er en international standard for kalibrering og test af pipetter samt andre stempeldrevne volumetriske instrumenter, POVA (Piston Operated Volumetric Apparatus , s\u00e5som buretter, dilutere, dispensere og laboratoriespr\u00f8jter.<\/p>\n\n\n\n


2. De ni dele af den nye ISO 8655:2022<\/strong><\/p>\n\n\n\n

Den opdaterede ISO 8655:2022 indeholder ni dele, hvilket er to flere end den tidligere version. De ni dele omhandler:<\/p>\n\n\n\n

    \n
  1. Terminologi, generelle krav og brugeranbefalinger<\/li>\n\n\n\n
  2. Pipetter<\/li>\n\n\n\n
  3. Buretter<\/li>\n\n\n\n
  4. Dilutere<\/li>\n\n\n\n
  5. Dispensere<\/li>\n\n\n\n
  6. Gravimetrisk reference kalibreringsprocedure til bestemmelse af volumen<\/li>\n\n\n\n
  7. Alternative kalibreringsprocedurer til bestemmelse af volumen<\/li>\n\n\n\n
  8. Fotometrisk reference kalibreringsprocedure til bestemmelse af volumen<\/li>\n\n\n\n
  9. Manuelt betjente pr\u00e6cisionslaboratoriespr\u00f8jter<\/li>\n<\/ol>\n\n\n\n

    <\/p>\n\n\n\n

    3. Strammere krav til pipetter i Del 2<\/strong><\/p>\n\n\n\n

    ISO 8655 Del 2 fasts\u00e6tter ISO 8655 max. tilladte fejl for stempelpipetter, krav til m\u00e6rkning samt den n\u00f8dvendige information fra producenterne. Den nye ISO forholder sig altid til at betragte pipette og spids som \u00e9t system, og pipettespidsproducenter skal sikre, at deres system opfylder alle krav. Maksimalt tilladte fejl angives i den nye opdatering som systematisk fejl, A% og tilf\u00e6ldig fejl, CV% og kun i 10, 50 og 100% af max. pipettevolumen.<\/p>\n\n\n\n


    4. Kalibrering og testning af pipetter i Del 6, Del 7 og Del 8<\/strong><\/strong><\/p>\n\n\n\n

    Kalibrering danner grundlaget for p\u00e5lidelig og reproducerbar pipettering. F\u00f8r opdateringen var den gravimetriske metode \u201dguldstandarden\u201d for kalibrering og test. If\u00f8lge den nye ISO 8655:2022 er det acceptabelt at bruge enten den gravimetriske metode (Del 6) eller den fotometriske metode (Del 8).
    Del 7 omhandler de strenge milj\u00f8krav til test og kalibrering. Det er ikke altid muligt at opfylde milj\u00f8krav ved kalibrering hos kunden. Alternative kalibreringsprocedurer er beskrevet i Del 7 og tillader afvigelser fra milj\u00f8krav indenfor definerede gr\u00e6nser. Kalibrering iht. den gravimetriske metode kan ogs\u00e5 udf\u00f8res iht. Del 7 A.2. Del 7 er bl.a. mere fleksibel mht. valg af testvolumener, og antal gentagelser pr. testvolumen.<\/p>\n\n\n\n


    5. Krav til pr\u00e6cisionsv\u00e6gte<\/strong><\/p>\n\n\n\n

    Den nye ISO har definereret krav til v\u00e6gte, der bruges til pipettekalibrering. Bl.a. til v\u00e6gtens opl\u00f8sning (deling), repeterbarhed samt til den ekspanderede m\u00e5leusikkerhed. I henhold til de nye ISO 8655-standarder skal nogle laboratorier muligvis opgradere deres v\u00e6gte. Der er nu behov for en 6-decimalersv\u00e6gt (0,000001 g) til kalibrering af pipetter<\/a> med nominelle volumener mindre end 20 \u00b5l, og kalibrering af pr\u00e6cisionsv\u00e6gte<\/a> for volumener mellem 20 \u00b5l og 200 \u00b5l.<\/p>\n\n\n\n

    Yderligere er kalibrering af multikanalspipetter nu specifikt n\u00e6vnt i den nye ISO. Vi anbefaler v\u00e6gte med flere vejeceller, s\u00e5som SpeedCal multikanalsv\u00e6gt fra Sartorius, der kan h\u00e5ndtere udvejning af multikanalspipetter op til 12 kanaler, parallelt.<\/p>\n\n\n\n

    Forfattet af:<\/strong> Tove S\u00f8gaard<\/p>\n","protected":false},"excerpt":{"rendered":"

    Sartorius har samlet fem vigtige punkter omkring de seneste \u00e6ndringer i pipettekalibreringsstandarderne, som defineres i ISO 8655:2022. Disse opdateringer er fra april 2022. 1. Hvad er ISO 8655-standarden? Pipetters n\u00f8jagtighed er helt afg\u00f8rende. ISO 8655 er en international standard for kalibrering og test af pipetter samt andre stempeldrevne volumetriske instrumenter, POVA (Piston Operated Volumetric Apparatus…<\/p>\n","protected":false},"author":5,"featured_media":3136,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[1],"tags":[2373,2375,1644],"class_list":["post-8458","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-uncategorized","tag-iso-standard","tag-kalibrering","tag-pipette"],"acf":[],"yoast_head":"\nVigtigt at vide om den nye pipette kalibreringsstandard ISO 8655:2022 - Dandiag (Demo)<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/demo.hviiddigital.dk\/dandiag.dk\/vigtigt-at-vide-om-den-nye-pipette-kalibreringsstandard-iso-86552022\/\" \/>\n<meta property=\"og:locale\" content=\"da_DK\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Vigtigt at vide om den nye pipette kalibreringsstandard ISO 8655:2022 - Dandiag (Demo)\" \/>\n<meta property=\"og:description\" content=\"Sartorius har samlet fem vigtige punkter omkring de seneste \u00e6ndringer i pipettekalibreringsstandarderne, som defineres i ISO 8655:2022. 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